Journal: PeerJ

Article Title: Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming

doi: 10.7717/peerj.3174

Figure Lengend Snippet: (A) REP-H103 expressed markers common to pluripotent cells including OCT4, SOX2, NANOG and TRA-1-60. (B) Pluripotency markers (OCT4, SOX2, NANOG and TRA-1-60) were not expressed in the parental cancer cell.

Article Snippet: Vectors pMX-based retroviral h Oct4 (Plasmid 17217) (Addgene, Cambridge, MA, USA), h Sox2 (Plasmid 17218) (Addgene, Cambridge, MA, USA), h Klf4 (Plasmid 17219) (Addgene, Cambridge, MA, USA), h c-Myc (Plasmid 17220) (Addgene, Cambridge, MA, USA), retroviral gag–pol packaging plasmid (Plasmid 8449) (Addgene, Cambridge, MA, USA), VSV-G expression plasmid (Plasmid 8454) (Addgene, Cambridge, MA, USA) and pMX-GFP (Cell Biolabs, San Diego, CA, USA) used in these experiments were provided by Dr. Shigeki Sugii, DUKE-NUS Graduate Medical School, Singapore.

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